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2024

A Novel Method for Real-Time Quantification of Radioligand Binding to Living Tumor Cells In Vitro

  • Bäck T, Albertsson P, Aneheim E, et al. ,

  • Cancer Biother Radiopharm ,

  • 2024,

  • Instrument : NucleoCounter® NC-200™
  • Zelltyp: Krebszellen
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Onkologie

Background: Real-time quantification of radioligand binding to cells under in vivo-like conditions improves evaluation of clinical potential. Materials and Methods: SKOV-3 tumor cells were grown in a monolayer on a thin glass plate placed in a sealable shallow chamber with...

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Cultivated Meat from Aligned Muscle Layers and Adipose Layers Formed from Glutenin Films

  • Yao Y, Yuen JSK Jr, Sylvia R, et al. ,

  • ACS Biomater Sci Eng ,

  • 2024,

  • Instrument : NucleoCounter® NC-200™
  • Zelltyp: Säugetierzellen
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Kulturfleisch

Cultivated meat production is a promising technology to generate meat while reducing the reliance on traditional animal farming. Biomaterial scaffolds are critical components in cultivated meat production, enabling cell adhesion, proliferation, differentiation, and orientation. In the present work, naturally derived...

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Extracellular vesicle biogenesis of three-dimensional human pluripotent stem cells in a novel Vertical-Wheel bioreactor

  • Muok L, Sun L, Esmonde C, et al. ,

  • Journal of Extracellular Biology ,

  • 2024,

  • 3(1):e133

  • Instrument : NucleoCounter® NC-200™
  • Zelltyp: iPSCs & ESCs
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Stammzellenforschung

Extracellular vesicles (EVs) secreted by human‐induced pluripotent stem cells (hiPSCs) have great potential as cell‐free therapies in various diseases, including prevention of blood–brain barrier senescence and stroke. However, there are still challenges in pre‐clinical and clinical use of hiPSC‐EVs due...

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2023

Benzophenone-3 exposure alters composition of tumor infiltrating immune cells and increases lung seeding of 4T1 breast cancer cells

  • Morin SM, Gregory KJ, Medeiros B, et al. ,

  • Advances in Cancer Biology - Metastasis ,

  • 2023,

  • 7:100080

  • Instrument : NucleoCounter® NC-202™
  • Zelltyp: Immunzellen
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Onkologie

Environmental chemicals are a persistent and pervasive part of everyday life. A subset of environmental chemicals are xenoestrogens, compounds that bind to the estrogen receptor (ER) and drive estrogen-related processes. One such chemical, benzophenone-3 (BP3), is a common chemical in...

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  • Instrument : NucleoCounter® NC-202™
  • Zelltyp: MSCs
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Stammzellenforschung

Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation, via electrospraying, for MSC expansion using a commercially available hydrogel that is durable, optimized...

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  • Instrument : NucleoCounter® NC-250™
  • Zelltyp: Krebszellen
  • Assay: Zellzyklus-Assay
  • Forschungsbereich: Onkologie

Diffuse large B-cell lymphoma (DLBCL) is an aggressive disease that exhibits constitutive activation of phosphoinositide 3-kinase (PI3K) driven by chronic B-cell receptor signaling or PTEN deficiency. Since pan-PI3K inhibitors cause severe side effects, we investigated the anti-lymphoma efficacy of the...

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