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2024

A Novel Method for Real-Time Quantification of Radioligand Binding to Living Tumor Cells In Vitro

  • Bäck T, Albertsson P, Aneheim E, et al. ,

  • Cancer Biother Radiopharm ,

  • 2024,

  • Instrument : NucleoCounter® NC-200™
  • Zelltyp: Krebszellen
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Onkologie

Background: Real-time quantification of radioligand binding to cells under in vivo-like conditions improves evaluation of clinical potential. Materials and Methods: SKOV-3 tumor cells were grown in a monolayer on a thin glass plate placed in a sealable shallow chamber with...

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Cultivated Meat from Aligned Muscle Layers and Adipose Layers Formed from Glutenin Films

  • Yao Y, Yuen JSK Jr, Sylvia R, et al. ,

  • ACS Biomater Sci Eng ,

  • 2024,

  • Instrument : NucleoCounter® NC-200™
  • Zelltyp: Säugetierzellen
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Kulturfleisch

Cultivated meat production is a promising technology to generate meat while reducing the reliance on traditional animal farming. Biomaterial scaffolds are critical components in cultivated meat production, enabling cell adhesion, proliferation, differentiation, and orientation. In the present work, naturally derived...

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Extracellular vesicle biogenesis of three-dimensional human pluripotent stem cells in a novel Vertical-Wheel bioreactor

  • Muok L, Sun L, Esmonde C, et al. ,

  • Journal of Extracellular Biology ,

  • 2024,

  • 3(1):e133

  • Instrument : NucleoCounter® NC-200™
  • Zelltyp: iPSCs & ESCs
  • Assay: Zellzahl-und-Viabilitäts-Assay
  • Forschungsbereich: Stammzellenforschung

Extracellular vesicles (EVs) secreted by human‐induced pluripotent stem cells (hiPSCs) have great potential as cell‐free therapies in various diseases, including prevention of blood–brain barrier senescence and stroke. However, there are still challenges in pre‐clinical and clinical use of hiPSC‐EVs due...

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2023

Odd- and even-numbered medium-chained fatty acids protect against glutathione depletion in very long-chain acyl-CoA dehydrogenase deficiency

  • Lund M, Heaton R, Hargreaves IP, et al. ,

  • Biochim Biophys Acta Mol Cell Biol Lipids ,

  • 2023,

  • 1868(2):159248

  • Instrument : NucleoCounter® NC-3000™
  • Zelltyp: Primärzellen
  • Assay: Zellvitalitäts-Assay
  • Forschungsbereich: Zellbiologie

Recent trials have reported the ability of triheptanoin to improve clinical outcomes for the severe symptoms associated with long-chain fatty acid oxidation disorders, including very long-chain acyl-CoA dehydrogenase (VLCAD) deficiency. However, the milder myopathic symptoms are still challenging to treat...

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  • Instrument : NucleoCounter® NC-3000™
  • Zelltyp: Krebszellen
  • Assay: Zellzahl-und-Viabilitäts-Assay, Zellzyklus-Assay
  • Forschungsbereich: Onkologie

The presence of di-(2-ethylhexyl) phthalate (DEHP) in the aquatic systems, specifically marine sediments has attracted considerable attention worldwide, as it enters the food chain and adversely affects the aquatic environment and subsequently human health. This study reports an efficient carbocatalytic...

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  • Instrument : NucleoCounter® NC-3000™
  • Zelltyp: Krebszellen
  • Assay: Annexin V-Assay, Zellzyklus-Assay
  • Forschungsbereich: Onkologie

Doxorubicin (DOX) is one of the most used chemotherapeutic agents in the treatment of various types of cancer. However, a continual problem that is associated with its application in therapeutic regimens is the development of dose-dependent cardiotoxicity. The progression of...

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